Journal: Immunity
Article Title: Tissue damage signaling is a prerequisite for protective neutrophil recruitment to microbial infection in zebrafish
doi: 10.1016/j.immuni.2018.04.020
Figure Lengend Snippet: (A) Cartoon scheme of experiment. (B) Ear injection of microbes generates a localized source of infection as visualized with EGFP-tagged PA (shown: hypotonic bathing conditions). Panel represents n=6 injection experiments. (C) Upper panel, time-lapse montage of neutrophil recruitment to infected ears in a Low Responder (left) and a High Responder (right) animal at indicated times. Panels represent n=205 (left) and n=125 (right) injection experiments, respectively. Neutrophils are in red. Fluorescent beads (blue) mark the ear region. Scale bar, 100 µm. Lower panel, neutrophil recruitment kinetics in Hypo + PA fish computationally classified into low (black) and high (red) responders by comparison to user-generated training sets. High responder index is depicted as pie chart. Blue percentage, an unsupervised (that is, no user-defined training sets) Gaussian distribution clustering algorithm determines a similar HR-index. Parentheses, number of injection experiments. Please see also Figure S1.
Article Snippet: User-unsupervised, Gaussian mixture distribution model clustering ( McLachlan and Peel, 2000 ) into two clusters using the Matlab ‘fitgmdist’ command gave largely similar results ( Figure S1B ).
Techniques: Injection, Infection, Comparison, Generated